Genetic Engineering Synthesis Essay
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Genetic engineering as the direct manipulation of DNA by humans outside breeding and mutations has only existed since the 1970s. In 1972, Paul Berg created the first recombinant DNA molecules by combining DNA from the monkey virus SV40 with that of the lambda virus. The first field trials of genetically engineered plants occurred in France and the US in 1986, tobacco plants were engineered to be resistant to herbicides.
It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA is obtained by either isolating and copying the genetic material of interest using recombinant DNA methods or by artificially synthesising the DNA. Used in research and industry, genetic engineering has been applied to the production of cancer therapies, brewing yeasts, genetically modified plants and livestock, and more.
Genetic engineering, also called genetic modification or genetic manipulation, is the modification and manipulation of an organism's genes using technology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA is obtained by either isolating and copying the genetic material of interest using recombinant DNA methods or by artificially synthesising the DNA. A construct is usually created and used to insert this DNA into the host organism. The first recombinant DNA molecule was made by Paul Berg in 1972 by combining DNA from the monkey virus SV40 with the lambda virus. As well as inserting genes, the process can be used to remove, or "knock out", genes. The new DNA can be inserted randomly, or targeted to a specific part of the genome.[1]
An organism that is generated through genetic engineering is considered to be genetically modified (GM) and the resulting entity is a genetically modified organism (GMO). The first GMO was a bacterium generated by Herbert Boyer and Stanley Cohen in 1973. Rudolf Jaenisch created the first GM animal when he inserted foreign DNA into a mouse in 1974. The first company to focus on genetic engineering, Genentech, was founded in 1976 and started the production of human proteins. Genetically engineered human insulin was produced in 1978 and insulin-producing bacteria were commercialised in 1982. Genetically modified food has been sold since 1994, with the release of the Flavr Savr tomato. The Flavr Savr was engineered to have a longer shelf life, but most current GM crops are modified to increase resistance to insects and herbicides. GloFish, the first GMO designed as a pet, was sold in the United States in December 2003. In 2016 salmon modified with a growth hormone were sold.
Genetic engineering has been applied in numerous fields including research, medicine, industrial biotechnology and agriculture. In research, GMOs are used to study gene function and expression through loss of function, gain of function, tracking and expression experiments. By knocking out genes responsible for certain conditions it is possible to create animal model organisms of human diseases. As well as producing hormones, vaccines and other drugs, genetic engineering has the potential to cure genetic diseases through gene therapy. The same techniques that are used to produce drugs can also have industrial applications such as producing enzymes for laundry detergent, cheeses and other products.
Genetic engineering is a process that alters the genetic structure of an organism by either removing or introducing DNA, or modifying existing genetic material in situ. Unlike traditional animal and plant breeding, which involves doing multiple crosses and then selecting for the organism with the desired phenotype, genetic engineering takes the gene directly from one organism and delivers it to the other. This is much faster, can be used to insert any genes from any organism (even ones from different domains) and prevents other undesirable genes from also being added.[4]
Genetic engineering could potentially fix severe genetic disorders in humans by replacing the defective gene with a functioning one.[5] It is an important tool in research that allows the function of specific genes to be studied.[6] Drugs, vaccines and other products have been harvested from organisms engineered to produce them.[7] Crops have been developed that aid food security by increasing yield, nutritional value and tolerance to environmental stresses.[8]
Genetic engineering does not normally include traditional breeding, in vitro fertilisation, induction of polyploidy, mutagenesis and cell fusion techniques that do not use recombinant nucleic acids or a genetically modified organism in the process.[9] However, some broad definitions of genetic engineering include selective breeding.[10] Cloning and stem cell research, although not considered genetic engineering,[11] are closely related and genetic engineering can be used within them.[12] Synthetic biology is an emerging discipline that takes genetic engineering a step further by introducing artificially synthesised material into an organism.[13]
Plants, animals or microorganisms that have been changed through genetic engineering are termed genetically modified organisms or GMOs.[14] If genetic material from another species is added to the host, the resulting organism is called transgenic. If genetic material from the same species or a species that can naturally breed with the host is used the resulting organism is called cisgenic.[15] If genetic engineering is used to remove genetic material from the target organism the resulting organism is termed a knockout organism.[16] In Europe genetic modification is synonymous with genetic engineering while within the United States of America and Canada genetic modification can also be used to refer to more conventional breeding methods.[17][18][19]
In 1972, Paul Berg created the first recombinant DNA molecules by combining DNA from the monkey virus SV40 with that of the lambda virus.[26] In 1973 Herbert Boyer and Stanley Cohen created the first transgenic organism by inserting antibiotic resistance genes into the plasmid of an Escherichia coli bacterium.[27][28] A year later Rudolf Jaenisch created a transgenic mouse by introducing foreign DNA into its embryo, making it the world's first transgenic animal[29] These achievements led to concerns in the scientific community about potential risks from genetic engineering, which were first discussed in depth at the Asilomar Conference in 1975. One of the main recommendations from this meeting was that government oversight of recombinant DNA research should be established until the technology was deemed safe.[30][31]
In 1976 Genentech, the first genetic engineering company, was founded by Herbert Boyer and Robert Swanson and a year later the company produced a human protein (somatostatin) in E. coli. Genentech announced the production of genetically engineered human insulin in 1978.[32] In 1980, the U.S. Supreme Court in the Diamond v. Chakrabarty case ruled that genetically altered life could be patented.[33] The insulin produced by bacteria was approved for release by the Food and Drug Administration (FDA) in 1982.[34]
The next step is to isolate the candidate gene. The cell containing the gene is opened and the DNA is purified.[53] The gene is separated by using restriction enzymes to cut the DNA into fragments[54] or polymerase chain reaction (PCR) to amplify up the gene segment.[55] These segments can then be extracted through gel electrophoresis. If the chosen gene or the donor organism's genome has been well studied it may already be accessible from a genetic library. If the DNA sequence is known, but no copies of the gene are available, it can also be artificially synthesised.[56] Once isolated the gene is ligated into a plasmid that is then inserted into a bacterium. The plasmid is replicated when the bacteria divide, ensuring unlimited copies of the gene are available.[57] The RK2 plasmid is notable for its ability to replicate in a wide variety of single-celled organisms, which makes it suitable as a genetic engineering tool.[58]
Genetic engineering has applications in medicine, research, industry and agriculture and can be used on a wide range of plants, animals and microorganisms. Bacteria, the first organisms to be genetically modified, can have plasmid DNA inserted containing new genes that code for medicines or enzymes that process food and other substrates.[81][82] Plants have been modified for insect protection, herbicide resistance, virus resistance, enhanced nutrition, tolerance to environmental pressures and the production of edible vaccines.[83] Most commercialised GMOs are insect resistant or herbicide tolerant crop plants.[84] Genetically modified animals have been used for research, model animals and the production of agricultural or pharmaceutical products. The genetically modified animals include animals with genes knocked out, increased susceptibility to disease, hormones for extra growth and the ability to express proteins in their milk.[85]
Genetic engineering has many applications to medicine that include the manufacturing of drugs, creation of model animals that mimic human conditions and gene therapy. One of the earliest uses of genetic engineering was to mass-produce human insulin in bacteria.[32] This application has now been applied to human growth hormones, follicle stimulating hormones (for treating infertility), human albumin, monoclonal antibodies, antihemophilic factors, vaccines and many other drugs.[86][87] Mouse hybridomas, cells fused together to create monoclonal antibodies, have been adapted through genetic engineering to create human monoclonal antibodies.[88] Genetically engineered viruses are being developed that can still confer immunity, but lack the infectious sequences.[89]
Genetic engineering is also used to create animal models of human diseases. Genetically modified mice are the most common genetically engineered animal model.[90] They have been used to study and model cancer (the oncomouse), obesity, heart disease, diabetes, arthritis, substance abuse, anxiety, aging and Parkinson disease.[91] Potential cures can be tested against these mouse models. 2b1af7f3a8
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Great insights on genetic engineering and its potential in today’s world! It’s always fascinating to explore how science evolves and influences various fields. For students working on essays or assignments, especially when dealing with data analysis or complex topics, statistics assignment help can be incredibly useful in ensuring your research is accurate and well-supported.
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若元錠、表飛鳴、愛表斯、綠藻錠，熱門整腸保健品
有腸胃困擾的人，肯定對WAKAMOTO、表飛鳴和愛表斯不陌生，到底這些整腸藥物差在哪？是否真能幫助排便？網路謠傳吃綠藻錠有助排便，真是如此嗎？專家提醒，整腸藥物不是功能多就好，適合自己最重要！
這幾年赴日自助旅行的遊客越來越多，網路上也有很多人分享日本藥妝店必買清單、日本藥妝懶人包，其中，又以整腸保健品最熱門。究竟日本整腸藥是保健品，還是藥品？成分有什麼差別？對腸道健康有什麼影響？可以天天服用嗎？為此，特地專訪輔仁大學附設醫院藥師謝宜靜，來替讀者解答。
基本上，臺灣民眾最常買的日本整腸藥物有幾種，包含WAKAMOTO若元胃腸錠（又分為白色包裝和棕色包裝）、表飛鳴、愛表斯，雖然都屬於整腸保健品，但因成分不同，功效也略有差異，總體而言，可以簡單分成三類。
謝宜靜藥師說明，「WAKAMOTO若元整腸錠（白色包裝）」和「欣表飛鳴錠」富含益生菌，主打整腸效果，可以幫助重建胃腸道菌叢，改善腹瀉、便秘等問題；「愛表斯錠」含豐富啤酒酵母，主打營養補充的功能；至於WAKAMOTO若元錠胃腸錠（棕色包裝）同時綜合有「幫助消化」、「整腸」、「營養補充」三種功能。
啤酒酵母能改善消化不良，卻不是人人都適合
若細看成分，可發現「愛表斯錠」每錠劑（250mg）中含乾燥酵母（啤酒酵母）237.50mg，天然啤酒酵母含豐富的維生素B1、B2、B6、胺基酸、礦物質和食物纖維等多種營養成分，除了可以補充營養外，也有益於乳酸菌等益生菌生長，能改善食慾不振、消化不良。
市面上有許多含有啤酒酵母的保健品，謝宜靜藥師表示，啤酒酵母就是釀造啤酒時所使用的酵母菌。通常這些酵母菌經過5至10次的發酵後，會漸漸失去活性，無法繼續使用於啤酒發酵，市面上買到含有「啤酒酵母」的營養補充品，就是這些失去活性而退役的酵母菌，經乾燥處理後製成的產品。
雖然直接購買啤酒酵母保健食品對於腸胃保健能達到一定的功效，但謝宜靜藥師提醒，部分啤酒酵母商品強調其產品為「活酵母」，然而，釀酒酵母菌並不是人體原生菌種，直接使用活菌可能增加感染風險，因此，使用啤酒酵母製品應以死菌製成之酵母粉為宜。
謝宜靜藥師強調，雖然天然啤酒酵母看起來益處多，但使用時有3大注意事項：
1.啤酒酵母屬於高蛋白質的食物，肝、腎功能較差的民眾需小心食用，以免增加身體負擔，使病情惡化。
2.啤酒酵母富含核酸，痛風的病人應避免使用。
3.啤酒酵母的磷含量較高，高量的磷會導致鈣流失，補充時最好增加鈣質攝取。為了避免鈣磷比不平衡，不建議幼兒食用此類高磷食物。
欣表飛鳴、白色包裝若元錠 可補充益生菌，發揮整腸效果
謝宜靜藥師說，「欣表飛鳴錠」中每錠含濃縮的比菲德氏菌（Bifidobacterium bifidum）、糞鏈球菌（streptococcus faecalis）、嗜酸乳酸桿菌（Lactobacillus acidophilus）粉末各2mg。其中，比菲德氏菌主要分佈於大腸，可產生乳酸及醋酸，提高整腸效果；糞鏈球菌和嗜酸乳酸桿菌主要分佈於小腸，前者能迅速增殖，使雜亂的腸內菌叢正常化，後者可產生大量的乳酸，抑制有害菌。廠商期望透過三種益生菌，從小腸到大腸，發揮全腸道的整腸效果。
WAKAMOTO若元錠整腸錠（白色包裝）主成分為龍根菌（B. longum）、比菲德氏菌（B. bifidum）、嗜酸乳酸桿菌（L. acidophilus），其中龍根菌與比非德氏菌為「大腸」中的定殖菌種，分泌乳酸與醋酸抑制壞菌增生。而嗜酸乳酸桿菌則是長駐在「小腸」中，也能分泌乳酸與醋酸，抑制壞菌，並能增強免疫力、使腸道菌叢正常化。廠商同樣期望透過三種益生菌，從小腸到大腸，發揮全腸道的整腸效果。適合壓力大、吃飯不定時的上班族，因長期外食造成腸胃不適、腹瀉、腹脹、便秘者。
謝宜靜藥師補充，欣表飛鳴和WAKAMOTO若元胃腸錠（白色包裝）成分類似，都是益生菌，但要注意這兩種產品皆屬於醫師、藥師、藥劑師指示藥品，適合腸胃出現狀況的人在醫師及藥師的輔導下使用，若沒有上述情況卻自行服用或長期持續使用，可能破壞腸內益生菌自動平衡的能力。
另外，益生菌並不是對每個人都有益處，因為益生菌屬活菌，用於某些族群，如免疫不全的病人、早產兒、中心靜脈導管置入、腸黏膜受損的患者等，可能會引發危險，臨床上曾有引起敗血癥等併發症的報告。
棕色包裝若元胃腸錠，能助消化、整腸、補充營養
而最多功能的WAKAMOTO若元胃腸錠（棕色包裝）主成分為米麴菌（含有6種消化酵素）、啤酒酵母粉末（維生素加強並富含胺基酸、多種礦物質）、乳酸菌（糞鏈球菌streptococcus faecalis）。其中，乳酸菌（S. faecalis）能抑制引起胃腸疾患的有害細菌繁殖，調整腸道機能，改善便秘、下痢、消化不良等症狀，因此可以說具有「幫助消化」、「整腸」、「補充營養」三種功能，適合胃腸功能較退化的銀髮族或因腸胃耗弱而引起的消化不良、食慾不振、腹脹便秘者。
不過，謝宜靜藥師指出，WAKAMOTO若元胃腸錠（棕色包裝）雖然功能較多，但在單一功能的劑量上卻較其他產品少。由於益生菌含量較少，因此「整腸」功能不如欣表飛鳴及WAKAMOTO若元整腸錠（白色包裝）；而就「營養補充」層面，成人一日劑量27顆中，酵母的總含量是2490.1mg，不及愛表斯錠一日成人劑量30顆裡酵母的總含量7125mg，所含的營養素種類也比較少。因此，整腸藥物不是功能越多越好，而是要挑選最適合自己的。
例如，若是想要促進食慾、補充營養，謝宜靜藥師建議，可考慮選擇愛表斯錠，缺點是一天需要吃30顆，有些胃口小的人，光吃完藥跟喝完水就沒有胃口了。若需要益生菌的話，可考慮選擇欣表飛鳴錠或WAKAMOTO整腸藥（白色包裝）。不過，要特別提醒，身體健康的人並不需要特別服藥補充益生菌，因為腸內乳酸菌可自動保持平衡的狀態，並發揮良好的作用。只有身體不適，或因飲食、環境變化而水土不服、壓力大、高齡化等原因造成劣菌增殖、腸內菌叢失去平衡，而出現腹瀉、便秘等情況，才需要補充乳酸菌以保持正常的狀態。
謝宜靜藥師特別提醒，若沒有這些症狀不建議長期持續使用，以免破壞腸內益生菌自動平衡的能力。而在挑選整腸劑之前，應先向專業的醫師及藥師諮詢，尤其慢性病患者、孕婦、嬰幼兒及老年人用藥禁忌較多，在使用整腸藥物前一定要記得諮詢醫師或藥師的意見。
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TOKYO MOHOO BOX 換彈式電子煙評價
隨著電子煙市場的快速發展，越來越多的品牌和產品涌現出來。其中，TOKYO MOHOO BOX 換彈式電子煙因其獨特的設計和便捷的使用體驗，受到了廣大用戶的關注和喜愛。那麼，TOKYO MOHOO換彈式電子煙到底好抽嗎？本文將從多個角度進行評價，幫助您了解這款產品的優缺點。
1. 外觀設計
東京魔盒換彈式電子煙採用簡約而時尚的設計，外觀小巧輕便，方便攜帶。其流線型的機身和多種顏色選擇，使其既適合日常使用，也能成為時尚配件。
TOKYO MOHOO BOX 換彈式電子煙評價
2. 換彈系統
這款魔盒電子煙最大的特色之一是其便捷的換彈系統。用戶只需輕輕一推，即可更換煙彈，無需繁瑣的操作。這種設計不僅提高了使用效率，還避免了傳統電子煙加油的麻煩。
3. 口感體驗
TOKYO MOHOO BOX 提供多種口味的煙彈選擇，包括經典煙草、薄荷、水果等多種口味。根據用戶反饋，這款電子煙的煙霧量適中，口感順滑，能夠滿足不同用戶的需求。特別是其煙草口味，受到了許多傳統吸煙者的好評。
4. 電池續航
這款電子煙內置高效鋰電池，充電速度快，續航能力強。根據用戶反饋，滿電狀態下可持續使用一天以上，適合長時間外出使用。
TOKYO MOHOO BOX 換彈式電子煙評價
5. 性價比
相比市場上的其他電子煙產品，TOKYO MOHOO BOX 換彈式電子煙的價格相對親民，且煙彈更換方便，綜合來看性價比較高。對於想要嘗試電子煙的新手以及尋求高性價比產品的用戶來說，這是一個不錯的選擇。
結論
總的來說，TOKYO MOHOO BOX 電子煙在外觀設計、換彈系統、口感體驗和電池續航方面都有不錯的表現。其便捷的使用方式和多樣的口味選擇，使其成為市場上備受關注的產品之一。
如果您正在尋找一款高性價比且使用方便的電子煙，東京魔盒MOHOO BOX 無疑是一個值得考慮的選擇。
—END—
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แทงบอลออนไลน์ การพนันรูปแบบกีฬาที่ได้รับความนิยมเป็นอันดับ 1 เพราะสามารถเดิมพันได้ง่าย ๆ ผ่านโทรศัพท์มือถือเครื่องเดียว แค่เป็นสมาชิกเว็บไซต์ UFABET เท่านี้คุณก็แทงบอลได้แล้ว ต่อไปคุณไม่ต้องไปเดิมพันตามโต๊ะหรือฝากใครเดิมพันอีกต่อไปแล้ว เพราะ UFABET ได้ปรับเปลี่ยนการเดิมพันมาไว้ในรูปแบบออนไลน์ คุณสามารถเดิมพันกันได้ง่ายมากขึ้น อีกทั้งยังสะดวกสบาย และประหยัดเวลาของคุณไปได้เยอะเลยทีเดียว และถ้าคุณสนใจเป็นสมาชิกกับเราวันนี้ คุณจะได้รับสิทธิพิเศษอีกมากมายเพิ่มไปอีกด้วย เรามอบให้คุณไปเลยแบบฟรี ๆ อย่ารอช้า มาเป็นสมาชิกกับเราได้เลยตอนนี้ เราเปิดให้สมัครสมาชิกฟรี